منابع مشابه
Hi-C in Budding Yeast.
Hi-C enables simultaneous detection of interaction frequencies between all possible pairs of restriction fragments in the genome. The Hi-C method is based on chromosome conformation capture (3C), which uses formaldehyde cross-linking to fix chromatin regions that interact in three-dimensional space, irrespective of their genomic locations. In the Hi-C protocol described here, cross-linked chrom...
متن کاملRNAi in budding yeast.
RNA interference (RNAi), a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast Saccharomyces cerevisiae. Here, we show that RNAi is present in other budding yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate small interfering ...
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We describe methods for live-cell imaging of yeast cells that we have exploited to image yeast polarity establishment. As a rare event occurring on a fast time-scale, imaging polarization involves a trade-off between spatiotemporal resolution and long-term imaging without excessive phototoxicity. By synchronizing cells in a way that increases resistance to photodamage, we discovered unexpected ...
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A complex signalling network governs the response of Saccharomyces cerevisiae to an array of environmental stimuli and stresses. In the present article, we provide an overview of the main signalling system and discuss the mechanisms by which yeast integrates and separates signals from these sources. We apply our classification scheme to a simple semi-quantitative model of the HOG (high-osmolari...
متن کاملChromosome associations in budding yeast
The distribution of chromosomes within interphase nuclei is not random. First, in most species there exists a polarized arrangement of chromosome arms, the so-called Rabl orientation, where centromeres are assembled near one pole of the nucleus, while telomeres point towards the opposite pole [for examples from various organisms, see the following references (Foe and Alberts, 1985; Fussell, 198...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2015
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.prot085209